The Volume Calculator plugin is designed to let users measure the volumes of arbitrary segments of blood vessels by selecting them directly from a 3D image. Although Volume Calculator was developed for quantification in a mouse hindlimb ischemia experiment, it should be useful for the volume measurement of any network. This possibility is determined by the following requirement: the plugin is configured to work best with images that can be read and analyzed by the Skeleton 3D and Analyze 3D ...